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RAE1 gamma Monoclonal Antibody (CX1), PE, eBioscience

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RAE1 gamma Monoclonal Antibody (CX1), PE, eBioscience

RAE1 gamma Monoclonal Antibody (CX1), PE, eBioscience

PRODUCT DETAILS

Host: Rat

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: CX1

Format: PE

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 0.125 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS


Specific Information

Description: The CX1 monoclonal antibody reacts with the mouse retinoic acid early inducible gamma (RAE-1gamma) and weakly cross-reacts with the RAE-1alpha and beta, but not with delta and ε. These 5 members of the RAE-1 family, along with another protein encoded by the minor histocompatibility gene complex called H60 have been identified as ligands for the mouse NKG2D molecule. These ligands are normally absent from adult tissue, however the presence of retinoic acid or certain disease conditions (such as tumors) can induce up-regulation of this protein at the cell surface. RAE-1alpha, -beta, and -gamma and H60 are expressed in BALB/c mice, but not in C57BL/6, whereas RAE-1delta, and -ε are expressed in C57BL/6 mice. It is reported that in NOD mice, both NKG2D and its ligand RAE-1 are expressed on the same NK cells, resulting in self-modulation of NKG2D expression and function.

Applications Reported: The CX1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The CX1 antibody has been tested by flow cytometric analysis of YAC-1 cell line and mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
$46.50

Original: $155.00

-70%
RAE1 gamma Monoclonal Antibody (CX1), PE, eBioscience

$155.00

$46.50

Product Information

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Description

PRODUCT DETAILS

Host: Rat

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: CX1

Format: PE

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 0.125 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS


Specific Information

Description: The CX1 monoclonal antibody reacts with the mouse retinoic acid early inducible gamma (RAE-1gamma) and weakly cross-reacts with the RAE-1alpha and beta, but not with delta and ε. These 5 members of the RAE-1 family, along with another protein encoded by the minor histocompatibility gene complex called H60 have been identified as ligands for the mouse NKG2D molecule. These ligands are normally absent from adult tissue, however the presence of retinoic acid or certain disease conditions (such as tumors) can induce up-regulation of this protein at the cell surface. RAE-1alpha, -beta, and -gamma and H60 are expressed in BALB/c mice, but not in C57BL/6, whereas RAE-1delta, and -ε are expressed in C57BL/6 mice. It is reported that in NOD mice, both NKG2D and its ligand RAE-1 are expressed on the same NK cells, resulting in self-modulation of NKG2D expression and function.

Applications Reported: The CX1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The CX1 antibody has been tested by flow cytometric analysis of YAC-1 cell line and mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
RAE1 gamma Monoclonal Antibody (CX1), PE, eBioscience | Cytek Biosciences