NKG2AB6 Monoclonal Antibody (16a11), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: 16a11
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 16a11 monoclonal antibody reacts with the mouse NKG2A^B6. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD95 and are responsible for recognition of non-classical MHC class I antigen Qa-1. This antibody reacts with the C57Bl/6 mouse strain, but does not react with the BALB/c or 129 mouse strains.
When co-staining with NKG2A/C/E clone 20d5, it is important to stain for NKG2A^B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently.
Applications Reported: This 16a11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 16a11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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NKG2AB6 Monoclonal Antibody (16a11), PerCP-eFluor 710, eBioscience
NKG2AB6 Monoclonal Antibody (16a11), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: 16a11
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 16a11 monoclonal antibody reacts with the mouse NKG2A^B6. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD95 and are responsible for recognition of non-classical MHC class I antigen Qa-1. This antibody reacts with the C57Bl/6 mouse strain, but does not react with the BALB/c or 129 mouse strains.
When co-staining with NKG2A/C/E clone 20d5, it is important to stain for NKG2A^B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently.
Applications Reported: This 16a11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 16a11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $418.00
-70%$418.00
$125.40Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: 16a11
Format: PerCP-eFluor 710
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 16a11 monoclonal antibody reacts with the mouse NKG2A^B6. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD95 and are responsible for recognition of non-classical MHC class I antigen Qa-1. This antibody reacts with the C57Bl/6 mouse strain, but does not react with the BALB/c or 129 mouse strains.
When co-staining with NKG2A/C/E clone 20d5, it is important to stain for NKG2A^B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently.
Applications Reported: This 16a11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 16a11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
