Nanog Monoclonal Antibody (eBioMLC-51), Alexa Fluor 488, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: eBioMLC-51
Format: Alexa Fluor 488
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.06 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.
Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved.
Immunoblotting using eBioMLC51 reveals a band at ~ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen.
Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Product # 00-5523-00, for intracellular staining of mouse Nanog.
Applications Reported: This eBioMLC-51 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunocytochemistry.
Applications Tested: This eBioMLC-51 antibody has been tested by intracellular staining followed by flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to BestProtocols®: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This eBioMLC-51 antibody has been tested by immunocytochemistry on fixed and permeabilized F9 cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Nanog Monoclonal Antibody (eBioMLC-51), Alexa Fluor 488, eBioscience
Nanog Monoclonal Antibody (eBioMLC-51), Alexa Fluor 488, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: eBioMLC-51
Format: Alexa Fluor 488
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.06 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.
Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved.
Immunoblotting using eBioMLC51 reveals a band at ~ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen.
Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Product # 00-5523-00, for intracellular staining of mouse Nanog.
Applications Reported: This eBioMLC-51 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunocytochemistry.
Applications Tested: This eBioMLC-51 antibody has been tested by intracellular staining followed by flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to BestProtocols®: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This eBioMLC-51 antibody has been tested by immunocytochemistry on fixed and permeabilized F9 cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: eBioMLC-51
Format: Alexa Fluor 488
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.06 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.
Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved.
Immunoblotting using eBioMLC51 reveals a band at ~ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen.
Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Product # 00-5523-00, for intracellular staining of mouse Nanog.
Applications Reported: This eBioMLC-51 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunocytochemistry.
Applications Tested: This eBioMLC-51 antibody has been tested by intracellular staining followed by flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to BestProtocols®: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This eBioMLC-51 antibody has been tested by immunocytochemistry on fixed and permeabilized F9 cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
