IL-33R (ST2) Monoclonal Antibody (hIL33Rcap), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: hIL33Rcap
Format: PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This hIL33Rcap monoclonal antibody recognizes human IL-33R, also known as ST2. This antibody binds both, the membrane bound form (ST2L) and the soluble form (ST2S). This antibody is suitable for both surface and intracellular staining.
This hIL33Rcap clone is also used as a capture antibody in the IL-33R (ST2) Human ProcartaPlex™ Simplex Kit (Product # EPX010-12231-901)
Applications Reported: This hIL33Rcap antibody has been reported for use in flow cytometric analysis.
Applications Tested: This hIL33Rcap antibody has been pre-diluted and tested by flow cytometric analysis of LAD2 cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
IL-33R (ST2) Monoclonal Antibody (hIL33Rcap), PerCP-eFluor 710, eBioscience
IL-33R (ST2) Monoclonal Antibody (hIL33Rcap), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: hIL33Rcap
Format: PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This hIL33Rcap monoclonal antibody recognizes human IL-33R, also known as ST2. This antibody binds both, the membrane bound form (ST2L) and the soluble form (ST2S). This antibody is suitable for both surface and intracellular staining.
This hIL33Rcap clone is also used as a capture antibody in the IL-33R (ST2) Human ProcartaPlex™ Simplex Kit (Product # EPX010-12231-901)
Applications Reported: This hIL33Rcap antibody has been reported for use in flow cytometric analysis.
Applications Tested: This hIL33Rcap antibody has been pre-diluted and tested by flow cytometric analysis of LAD2 cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $497.00
-70%$497.00
$149.10Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: hIL33Rcap
Format: PerCP-eFluor 710
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This hIL33Rcap monoclonal antibody recognizes human IL-33R, also known as ST2. This antibody binds both, the membrane bound form (ST2L) and the soluble form (ST2S). This antibody is suitable for both surface and intracellular staining.
This hIL33Rcap clone is also used as a capture antibody in the IL-33R (ST2) Human ProcartaPlex™ Simplex Kit (Product # EPX010-12231-901)
Applications Reported: This hIL33Rcap antibody has been reported for use in flow cytometric analysis.
Applications Tested: This hIL33Rcap antibody has been pre-diluted and tested by flow cytometric analysis of LAD2 cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
