PRODUCT DETAILS

Host: Rat

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: JES6-5H4

Format: Biotin

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: Assay-dependent

Concentration: 0.5 mg/mL

Storage: 4°C, do not freeze

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: JES6-5H4 has been reported for use in detection of mouse IL-2 by ELISA, intracellular staining and flow cytometric analysis, immunoprecipitation, and for neutralizing IL-2 binding and biological activity.

Applications Tested: The JES6-5H4 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the purified JES6-1A12 (14-7022) antibody for capture and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.