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IgM Monoclonal Antibody (HIS40), FITC, eBioscience

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IgM Monoclonal Antibody (HIS40), FITC, eBioscience

IgM Monoclonal Antibody (HIS40), FITC, eBioscience

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: HIS40

Format: FITC

Reactivity: Rt

Application: Flow Cytometry

Tested Dilution: 0.25 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS


Specific Information

Description: The HIS40 monoclonal antibody reacts with the µ heavy chain of rat IgM. It does not react with other classes of rat immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated HIS40 is used as a detection secondary for rat IgM primary antibodies.

Applications Reported: The HIS40 antibody has been reported for use in flow cytometric analysis, immunocytochemistry and immunohistochemistry.

Applications Tested: The HIS40 antibody has been tested by flow cytometric analysis as a second step to detect rat IgM primary antibodies. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
$75.60

Original: $252.00

-70%
IgM Monoclonal Antibody (HIS40), FITC, eBioscience

$252.00

$75.60

Product Information

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Description

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: HIS40

Format: FITC

Reactivity: Rt

Application: Flow Cytometry

Tested Dilution: 0.25 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS


Specific Information

Description: The HIS40 monoclonal antibody reacts with the µ heavy chain of rat IgM. It does not react with other classes of rat immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated HIS40 is used as a detection secondary for rat IgM primary antibodies.

Applications Reported: The HIS40 antibody has been reported for use in flow cytometric analysis, immunocytochemistry and immunohistochemistry.

Applications Tested: The HIS40 antibody has been tested by flow cytometric analysis as a second step to detect rat IgM primary antibodies. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IgM Monoclonal Antibody (HIS40), FITC, eBioscience | Cytek Biosciences