EGR2 Monoclonal Antibody (erongr2), APC, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: erongr2
Format: APC
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20). Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells. Egr2 is predominantly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking. Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis. The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells. The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis.
Applications Reported: This erongr2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This erongr2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
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EGR2 Monoclonal Antibody (erongr2), APC, eBioscience
EGR2 Monoclonal Antibody (erongr2), APC, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: erongr2
Format: APC
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20). Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells. Egr2 is predominantly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking. Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis. The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells. The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis.
Applications Reported: This erongr2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This erongr2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $418.00
-70%$418.00
$125.40Product Information
Product Information
Shipping & Returns
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Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: erongr2
Format: APC
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20). Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells. Egr2 is predominantly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking. Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis. The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells. The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis.
Applications Reported: This erongr2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This erongr2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
