CD93 (AA4.1) Monoclonal Antibody (R139), eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b
Clonality: Monoclonal
Clone: R139
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody R139 recognizes human CD93, also known as C1qRp. The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A. CD93 is predicted to play a role in the clearance of apoptotic cells. Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells. Expression on DC's is downregulated upon maturation. Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors.
The monoclonal antibody R139 blocks C1q-mediated enhancement of phagocytosis.
CD93 can be shed from the cell surface. This phenomenon can be measured using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA. The epitope for R139 resides in the EGF domains.
Applications Reported: This R139 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, ELISA, and immunohistochemistry of formalin-fixed paraffin embedded tissue.
Applications Tested: This R139 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The R139 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 40 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
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CD93 (AA4.1) Monoclonal Antibody (R139), eBioscience
CD93 (AA4.1) Monoclonal Antibody (R139), eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b
Clonality: Monoclonal
Clone: R139
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody R139 recognizes human CD93, also known as C1qRp. The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A. CD93 is predicted to play a role in the clearance of apoptotic cells. Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells. Expression on DC's is downregulated upon maturation. Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors.
The monoclonal antibody R139 blocks C1q-mediated enhancement of phagocytosis.
CD93 can be shed from the cell surface. This phenomenon can be measured using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA. The epitope for R139 resides in the EGF domains.
Applications Reported: This R139 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, ELISA, and immunohistochemistry of formalin-fixed paraffin embedded tissue.
Applications Tested: This R139 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The R139 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 40 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b
Clonality: Monoclonal
Clone: R139
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The monoclonal antibody R139 recognizes human CD93, also known as C1qRp. The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A. CD93 is predicted to play a role in the clearance of apoptotic cells. Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells. Expression on DC's is downregulated upon maturation. Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors.
The monoclonal antibody R139 blocks C1q-mediated enhancement of phagocytosis.
CD93 can be shed from the cell surface. This phenomenon can be measured using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA. The epitope for R139 resides in the EGF domains.
Applications Reported: This R139 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, ELISA, and immunohistochemistry of formalin-fixed paraffin embedded tissue.
Applications Tested: This R139 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The R139 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 40 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
