CD45R (B220) Monoclonal Antibody (HIS24), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: HIS24
Format: PerCP-eFluor 710
Reactivity: Rt
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.
Applications Reported: This HIS24 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIS24 antibody has been tested by flow cytometric analysis of rat splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
CD45R (B220) Monoclonal Antibody (HIS24), PerCP-eFluor 710, eBioscience
CD45R (B220) Monoclonal Antibody (HIS24), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: HIS24
Format: PerCP-eFluor 710
Reactivity: Rt
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.
Applications Reported: This HIS24 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIS24 antibody has been tested by flow cytometric analysis of rat splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: HIS24
Format: PerCP-eFluor 710
Reactivity: Rt
Application: Flow Cytometry
Tested Dilution: 0.5 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.
Applications Reported: This HIS24 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIS24 antibody has been tested by flow cytometric analysis of rat splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
