CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), NovaFluor Blue 660-120S, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: eBioMWReg30 (MWReg30)
Format: NovaFluor Blue 660-120S
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.6 µg/test
Concentration: 0.1 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha Iib). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin beta III) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin.
Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This eBioMWReg30 (MWReg30) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioMWReg30 (MWReg30) antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue) Laser
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), NovaFluor Blue 660-120S, eBioscience
CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), NovaFluor Blue 660-120S, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: eBioMWReg30 (MWReg30)
Format: NovaFluor Blue 660-120S
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.6 µg/test
Concentration: 0.1 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha Iib). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin beta III) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin.
Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This eBioMWReg30 (MWReg30) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioMWReg30 (MWReg30) antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue) Laser
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $194.00
-70%$194.00
$58.20Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: eBioMWReg30 (MWReg30)
Format: NovaFluor Blue 660-120S
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.6 µg/test
Concentration: 0.1 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha Iib). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin beta III) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin.
Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This eBioMWReg30 (MWReg30) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioMWReg30 (MWReg30) antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue) Laser
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
