CD365 (TIM1) Monoclonal Antibody (1D12-TIM1), PE-Cyanine7, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 1D12-TIM1
Format: PE-Cyanine7
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 1D12-TIM1 monoclonal antibody recognizes human CD365 also known as Tim-1, Kim-1 or Havcr1. This antibody was generated using the immunoglobulin V domain of the extracellular part of the CD365 receptor, and is predicted to recognize all three haplotypes of human CD365. Clone 1D12-TIM1 has been shown to block entry of HCV virus into human hepatoma Huh7.5 cells in a dose dependent manner. It has been also shown to block the uptake of apoptotic cells by CD365-expressing 769-P cells.
Applications Reported: This 1D12-TIM1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 1D12-TIM1 antibody has been pre-diluted and tested by flow cytometric analysis of A549. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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CD365 (TIM1) Monoclonal Antibody (1D12-TIM1), PE-Cyanine7, eBioscience
CD365 (TIM1) Monoclonal Antibody (1D12-TIM1), PE-Cyanine7, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 1D12-TIM1
Format: PE-Cyanine7
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 1D12-TIM1 monoclonal antibody recognizes human CD365 also known as Tim-1, Kim-1 or Havcr1. This antibody was generated using the immunoglobulin V domain of the extracellular part of the CD365 receptor, and is predicted to recognize all three haplotypes of human CD365. Clone 1D12-TIM1 has been shown to block entry of HCV virus into human hepatoma Huh7.5 cells in a dose dependent manner. It has been also shown to block the uptake of apoptotic cells by CD365-expressing 769-P cells.
Applications Reported: This 1D12-TIM1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 1D12-TIM1 antibody has been pre-diluted and tested by flow cytometric analysis of A549. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $463.00
-70%$463.00
$138.90Product Information
Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 1D12-TIM1
Format: PE-Cyanine7
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.125 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 1D12-TIM1 monoclonal antibody recognizes human CD365 also known as Tim-1, Kim-1 or Havcr1. This antibody was generated using the immunoglobulin V domain of the extracellular part of the CD365 receptor, and is predicted to recognize all three haplotypes of human CD365. Clone 1D12-TIM1 has been shown to block entry of HCV virus into human hepatoma Huh7.5 cells in a dose dependent manner. It has been also shown to block the uptake of apoptotic cells by CD365-expressing 769-P cells.
Applications Reported: This 1D12-TIM1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 1D12-TIM1 antibody has been pre-diluted and tested by flow cytometric analysis of A549. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.











