CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), APC-eFluor 780, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: DTA-1
Format: APC-eFluor 780
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.
Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), APC-eFluor 780, eBioscience
CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), APC-eFluor 780, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: DTA-1
Format: APC-eFluor 780
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.
Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $449.00
-70%$449.00
$134.70Product Information
Product Information
Shipping & Returns
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Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: DTA-1
Format: APC-eFluor 780
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.
Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
