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CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio129c (129c)), Alexa Fluor 488, eBioscience

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CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio129c (129c)), Alexa Fluor 488, eBioscience

CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio129c (129c)), Alexa Fluor 488, eBioscience

PRODUCT DETAILS

Host: Rat

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: eBio129c (129c)

Format: Alexa Fluor 488

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 0.25 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS


Specific Information

Description: The eBio129c monoclonal antibody reacts with mouse PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by the antibody 120G8.

The epitope recognized by eBio129c is distinct from eBio927; thus, the antibodies can be used to costain, purify and identify pDCs.

Applications Reported: This eBio129c (129c) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio129c (129c) antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
$128.10

Original: $427.00

-70%
CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio129c (129c)), Alexa Fluor 488, eBioscience

$427.00

$128.10

Product Information

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Description

PRODUCT DETAILS

Host: Rat

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: eBio129c (129c)

Format: Alexa Fluor 488

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 0.25 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS


Specific Information

Description: The eBio129c monoclonal antibody reacts with mouse PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by the antibody 120G8.

The epitope recognized by eBio129c is distinct from eBio927; thus, the antibodies can be used to costain, purify and identify pDCs.

Applications Reported: This eBio129c (129c) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio129c (129c) antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio129c (129c)), Alexa Fluor 488, eBioscience | Cytek Biosciences