CD177 Monoclonal Antibody (MEM-166 (MEM166)), eFluor 660, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: MEM-166 (MEM166)
Format: eFluor 660
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.015 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64 kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug induced neutropenia, and graft failure following marrow transplantation.
Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MEM-166 (MEM166) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
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CD177 Monoclonal Antibody (MEM-166 (MEM166)), eFluor 660, eBioscience
CD177 Monoclonal Antibody (MEM-166 (MEM166)), eFluor 660, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: MEM-166 (MEM166)
Format: eFluor 660
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.015 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64 kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug induced neutropenia, and graft failure following marrow transplantation.
Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MEM-166 (MEM166) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: MEM-166 (MEM166)
Format: eFluor 660
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.015 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The MEM-166 monoclonal antibody reacts with human CD177, a 58-64 kDa GPI-anchored glycoprotein. The human CD177 locus codes for at least two alleles, NB1 and PRV-1, which differ by only 4 amino acids. CD177 is expressed on granulocytes, early erythroblasts, megakaryocytes, promyelocytes and myelocytes. CD177 mRNA levels are elevated in several different myeloproliferative disorders including polycythemia vera, and antibodies to CD177 are involved in neonatal alloimmune neutropenia, autoimmune drug induced neutropenia, and graft failure following marrow transplantation.
Applications Reported: This MEM-166 (MEM166) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MEM-166 (MEM166) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.











