CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 12-3A3-1B10
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.
Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested: This 12-3A3-1B10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Information
Product Information
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CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), eBioscience
CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 12-3A3-1B10
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.
Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested: This 12-3A3-1B10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $279.00
-70%$279.00
$83.70Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: 12-3A3-1B10
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.
Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested: This 12-3A3-1B10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
