c-MAF Monoclonal Antibody (sym0F1), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: sym0F1
Format: PerCP-eFluor 710
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 5 µL (0.0075 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.
Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This sym0F1 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.0075 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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c-MAF Monoclonal Antibody (sym0F1), PerCP-eFluor 710, eBioscience
c-MAF Monoclonal Antibody (sym0F1), PerCP-eFluor 710, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: sym0F1
Format: PerCP-eFluor 710
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 5 µL (0.0075 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.
Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This sym0F1 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.0075 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $508.00
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$152.40Product Information
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Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: sym0F1
Format: PerCP-eFluor 710
Reactivity: Hu, Ms
Application: Flow Cytometry
Tested Dilution: 5 µL (0.0075 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.
Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This sym0F1 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.0075 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
