PRODUCT DETAILS

Host: Armenian Hamster

Isotype: IgG

Clonality: Monoclonal

Clone: eBio299Arm

Format: Brilliant Violet 480

Reactivity: N/A

Application: Flow Cytometry

Tested Dilution: Assay-dependent

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.

Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use at the same concentration as the experimental antibody.

Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.

Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.